%0 Journal Article
%T SLFN5-mediated chromatin dynamics sculpt higher-order DNA repair topology.
%A Huang J
%A Wu C
%A Kloeber JA
%A Gao H
%A Gao M
%A Zhu Q
%A Chang Y
%A Zhao F
%A Guo G
%A Luo K
%A Dai H
%A Liu S
%A Huang Q
%A Kim W
%A Zhou Q
%A Zhu S
%A Wu Z
%A Tu X
%A Yin P
%A Deng M
%A Wang L
%A Yuan J
%A Lou Z
%J Mol Cell
%V 83
%N 7
%D 04 2023 6
%M 36854302
%F 19.328
%R 10.1016/j.molcel.2023.02.004
%X Repair of DNA double-strand breaks (DSBs) elicits three-dimensional (3D) chromatin topological changes. A recent finding reveals that 53BP1 assembles into a 3D chromatin topology pattern around DSBs. How this formation of a higher-order structure is configured and regulated remains enigmatic. Here, we report that SLFN5 is a critical factor for 53BP1 topological arrangement at DSBs. Using super-resolution imaging, we find that SLFN5 binds to 53BP1 chromatin domains to assemble a higher-order microdomain architecture by driving damaged chromatin dynamics at both DSBs and deprotected telomeres. Mechanistically, we propose that 53BP1 topology is shaped by two processes: (1) chromatin mobility driven by the SLFN5-LINC-microtubule axis and (2) the assembly of 53BP1 oligomers mediated by SLFN5. In mammals, SLFN5 deficiency disrupts the DSB repair topology and impairs non-homologous end joining, telomere fusions, class switch recombination, and sensitivity to poly (ADP-ribose) polymerase inhibitor. We establish a molecular mechanism that shapes higher-order chromatin topologies to safeguard genomic stability.