%0 Journal Article
%T Detection of Synthetic Cannabinoid CUMYL-PEGACLONE in E-Cigarette Oil and Hair.
%A Jiao TF
%A Li YQ
%A Kang G
%A Chen SS
%A Cheng LH
%J Fa Yi Xue Za Zhi
%V 38
%N 5
%D Oct 2022 25
%M 36727175
暂无%R 10.12116/j.issn.1004-5619.2022.320701
%X OBJECTIVE: To establish a combined high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) method to detect the synthetic cannabinoid CUMYL-PEGACLONE in e-cigarette oil and hair.
METHODS: HPLC-MS/MS and GC-MS were used to establish the detection method of CUMYL-PEGACLONE, and the hair of drug-involved persons and the seized e-cigarette oil were detected.
RESULTS: The main mass spectrometry characteristic ions m/z of CUMYL-PEGACLONE measured by GC-MS were 91, 179, 197, 254 and 372. CUMYL-PEGACLONE had a good linear relationship in the mass concentration range of 2-50 ng/mL, and the linear correlation coefficient (r) was greater than 0.99. The limit of detection (LOD) of CUMYL-PEGACLONE in hair was 0.01 ng/mg, and the limit of quantitation (LOQ) was 0.02 ng/mg. The LOD of CUMYL-PEGACLONE in e-cigarette oil was 1 ng/mg, and the LOQ was 2 ng/mg. The average recoveries of CUMYL-PEGACLONE under the attempt at high, intermediate and low levels in blank human hair and e-cigarette oil matrix were 98.2%-132.4% and 93.5%-110.6%, and the intraday and intraday precision were 1.2%-12.9% and 0.7%-2.9%. CUMYL-PEGACLONE was detected in the hair of 15 drug-involved persons. Except for 1 person who was lower than LOQ, the concentration of CUMYL-PEGACLONE in the hair of other 14 persons was 0.035-0.563 ng/mg. The mass fraction of CUMYL-PEGACLONE in 2 e-cigarette oil were 0.17% and 0.21%, respectively.
CONCLUSIONS: The established HPLC-MS/MS and GC-MS methods are applied to the detection of HPLC-MS/MS in drug-related cases, which provides strong evidence support for the handling authority to quickly investigate these cases, and also provides a reference for the identification of such substances in future.
目的: 建立联合高效液相色谱-串联质谱法(high performance liquid chromatography-tandem mass spectrometry,HPLC-MS/MS)和气相色谱-质谱法(gas chromatography-mass spectrometry,GC-MS)检测电子烟油和毛发中合成大麻素5-戊基-2-(2-苯基丙-2-基)-2,5-二氢-1H-吡啶[4,3-b]吲哚-1-酮(CUMYL-PEGACLONE)的方法。方法: 采用HPLC-MS/MS和GC-MS建立CUMYL-PEGACLONE检测方法,对涉毒人员毛发和查获的电子烟油进行检测。结果: GC-MS测得CUMYL-PEGACLONE主要质谱特征离子m/z为91、179、197、254和372;CUMYL-PEGACLONE在2~50 ng/mL质量浓度范围内线性关系良好,线性相关系数(r)大于0.99;毛发中CUMYL-PEGACLONE的HPLC-MS/MS方法检出限为0.01 ng/mg,定量限为0.02 ng/mg;电子烟油中CUMYL-PEGACLONE的HPLC-MS/MS方法检出限为1 ng/mg,定量限为2 ng/mg;CUMYL-PEGACLONE在空白毛发和电子烟油基质中高、中、低3个添加浓度下的回收率分别为98.2%~132.4%和93.5%~110.6%,日内精密度和日间精密度分别为1.2%~12.9%和0.7%~2.9%。专案中15例涉毒人员头发中均检出CUMYL-PEGACLONE,除1例低于定量限,另14例人员头发中CUMYL-PEGACLONE质量分数为0.035~0.563 ng/mg;2份电子烟油中CUMYL-PEGACLONE的质量分数分别为0.17%和0.21%。结论: 建立的HPLC-MS/MS和GC-MS方法应用于涉毒专案中CUMYL-PEGACLONE的检测,为办案机关快速侦办涉毒专案提供了有力证据支撑,亦为今后涉及此类物质的鉴定提供参考依据。.