%0 Journal Article
%T Liposaccharide-induced sustained mild inflammation fragments social behavior and alters basolateral amygdala activity.
%A Loh MK
%A Stickling C
%A Schrank S
%A Hanshaw M
%A Ritger AC
%A Dilosa N
%A Finlay J
%A Ferrara NC
%A Rosenkranz JA
%J Psychopharmacology (Berl)
%V 240
%N 3
%D Mar 2023
%M 36645464
%F 4.415
%R 10.1007/s00213-023-06308-8
%X <B>BACKGROUND: </B>Conditions with sustained low-grade inflammation have high comorbidity with depression and anxiety and are associated with social withdrawal. The basolateral amygdala (BLA) is critical for affective and social behaviors and is sensitive to inflammatory challenges. Large systemic doses of lipopolysaccharide (LPS) initiate peripheral inflammation, increase BLA neuronal activity, and disrupt social and affective measures in rodents. However, LPS doses commonly used in behavioral studies are high enough to evoke sickness syndrome, which can confound interpretation of amygdala-associated behaviors.<BR><B>OBJECTIVE: </B>The objectives of this study were to find a LPS dose that triggers mild peripheral inflammation but not observable sickness syndrome in adult male rats, to test the effects of sustained mild inflammation on BLA and social behaviors. To accomplish this, we administered single doses of LPS (0-100 μg/kg, intraperitoneally) and measured open field behavior, or repeated LPS (5 μg/kg, 3 consecutive days), and measured BLA neuronal firing, social interaction, and elevated plus maze behavior.<BR><B>RESULTS: </B>Repeated low-dose LPS decreased BLA neuron firing rate but increased the total number of active BLA neurons. Repeated low-dose LPS also caused early disengagement during social bouts and less anogenital investigation and an overall pattern of heightened social caution associated with reduced gain of social familiarity over the course of a social session.<BR><B>CONCLUSIONS: </B>These results provide evidence for parallel shifts in social interaction and amygdala activity caused by prolonged mild inflammation. This effect of inflammation may contribute to social symptoms associated with comorbid depression and chronic inflammatory conditions.