%0 Journal Article
%T Synthesis of Cy5-Labelled C5-Alkynyl-modified cytidine triphosphates via Sonogashira coupling for DNA labelling.
%A Kuznetsova VE
%A Shershov VE
%A Guseinov TO
%A Miftakhov RA
%A Solyev PN
%A Novikov RA
%A Levashova AI
%A Zasedatelev AS
%A Lapa SA
%A Chudinov AV
%J Bioorg Chem
%V 131
%N 0
%D 02 2023
%M 36528924
%F 5.307
%R 10.1016/j.bioorg.2022.106315
%X New applications of palladium-catalyzed Sonogashira-type cross-coupling reaction between C5-halogenated 2'-deoxycytidine-5'-monophosphate and novel cyanine dyes with a terminal alkyne group have been developed. The present methodology allows to synthesize of fluorescently labeled C5-nucleoside triphosphates with different acetylene linkers between the fluorophore and pyrimidine base in good to excellent yields under mild reaction conditions. Modified 2'-deoxycytidine-5'-triphosphates were shown to be good substrates for DNA polymerases and were incorporated into the DNA by polymerase chain reaction.