%0 Journal Article
%T Enhanced Yeast One-Hybrid Assays to Study Protein-DNA Interactions.
%A Berenson A
%A Fuxman Bass JI
%J Methods Mol Biol
%V 2599
%N 0
%D 2023
%M 36427139
暂无%R 10.1007/978-1-0716-2847-8_2
%X The specificity in gene regulation is controlled by interactions between transcription factors (TFs) and genomic DNA regions such as promoters and enhancers. Enhanced yeast one-hybrid (eY1H) assays are among the methods used for high-throughput detection of transcription factor-DNA interactions. Here, we describe the procedure for screening interactions between DNA regions of interest ("DNA-baits") and an array of transcription factors ("TF-preys"), after DNA-bait and TF-prey yeast strains have been generated. Using a high-density array robotic platform, this method can be used to screen interactions between multiple DNA regions and >1000 TFs within a single experiment.