%0 Journal Article
%T Cholesterol Chip for the Study of Cholesterol-Protein Interactions Using SPR.
%A He P
%A Faris S
%A Sagabala RS
%A Datta P
%A Xu Z
%A Callahan B
%A Wang C
%A Boivin B
%A Zhang F
%A Linhardt RJ
%J Biosensors (Basel)
%V 12
%N 10
%D Sep 2022 25
%M 36290926
%F 5.743
%R 10.3390/bios12100788
%X Cholesterol, an important lipid in animal membranes, binds to hydrophobic pockets within many soluble proteins, transport proteins and membrane bound proteins. The study of cholesterol-protein interactions in aqueous solutions is complicated by cholesterol's low solubility and often requires organic co-solvents or surfactant additives. We report the synthesis of a biotinylated cholesterol and immobilization of this derivative on a streptavidin chip. Surface plasmon resonance (SPR) was then used to measure the kinetics of cholesterol interaction with cholesterol-binding proteins, hedgehog protein and tyrosine phosphatase 1B.