%0 Journal Article
%T A novel fluorescence biosensor based on double-stranded DNA branch migration-induced HCR and DNAzyme feedback circuit for sensitive detection of Pseudomonas aeruginosa (clean version).
%A Xie Y
%A Xie G
%A Yuan J
%A Zhang J
%A Yang Y
%A Yao Y
%A Wu Y
%A Bai D
%A Chen K
%A Li B
%A Song L
%A Chen H
%A Xie Y
%A Xie G
%A Yuan J
%A Zhang J
%A Yang Y
%A Yao Y
%A Wu Y
%A Bai D
%A Chen K
%A Li B
%A Song L
%A Chen H
%J Anal Chim Acta
%V 1232
%N 0
%D Nov 2022 1
%M 36257735
%F 6.911
%R 10.1016/j.aca.2022.340449
%X Pseudomonas aeruginosa (P. aeruginosa) is one of the most common bacteria in nosocomial infection. Here, a novel fluorescence biosensor based on double-stranded DNA branch migration-induced hybridization chain reaction (HCR) and DNAzyme feedback circuit was constructed for sensitive detection of P. aeruginosa. The binding of P. aeruginosa with its aptamer on a DNA three-way junction structure initiated the double-stranded DNA branch migration to form two DNA "Y" junction structures. One DNA "Y" junction structure opened the fluorescence-labelled DNA hairpins and triggered the HCR. The other DNA "Y" junction structure formed a double-stranded DNAzyme and cleaved the specific ribonucleotide site, producing new triggering probes to start the next cycle of the double-stranded DNA branch migration. Ultimately, a large number of DNA "Y" junction structures were produced, which greatly promoted signal amplification. Under optimized conditions, the proposed biosensor detected a wide linearity range of 102-107 CFU mL-1, and the limit of detection was 37 CFU mL-1 (S/N = 3). The recovery test results indicated that the biosensor has promising clinical application potential. Because of the simultaneous initiation of the HCR and the DNAzyme feedback circuit through the double-stranded DNA branch migration, the constructed biosensor provided an ideal platform for pathogenic bacteria detection without protein enzymes and complex signal amplification procedures.