%0 Journal Article
%T New prenylated flavonoid glycosides derived from Epimedium wushanense by β-glucosidase hydrolysis and their testosterone production-promoting effects.
%A Sun XG
%A Pang X
%A Liang HZ
%A Zhang J
%A Wang B
%A Li Q
%A Wang J
%A Chen XJ
%A Guo BL
%A Ma BP
%A Sun XG
%A Pang X
%A Liang HZ
%A Zhang J
%A Wang B
%A Li Q
%A Wang J
%A Chen XJ
%A Guo BL
%A Ma BP
%J Chin J Nat Med
%V 20
%N 9
%D Sep 2022
%M 36162956
%F 3.887
%R 10.1016/S1875-5364(22)60188-2
%X Six new prenylated flavonoid glycosides, including four new furan-flavonoid glycosides wushepimedoside A-D (1-4) and two new prenyl flavonoid derivatives wushepimedoside E-F (5-6), and one know analog epimedkoreside B (7) were isolated from biotransformation products of the aerial parts of Epimedium wushanense. Their structures were elucidated according to comprehensive analysis of HR-MS and NMR spectroscopic data, and the absolute configurations were assigned using experimental and calculated electronic circular dichroism (ECD) data. The regulatory activity of compounds 1-7 on the production of testosterone in primary rat Leydig cells were investigated, and 4 and 5 exhibited testosterone production-promoting activities. Molecular docking analysis suggested that bioactive compounds 4 and 5 showed the stable binding with 3β-HSD and 4 also had good affinity with Cyp17A1, which suggested that these compounds may regulate testosterone production through stimulating the expression of the above two key proteins.