%0 Journal Article
%T Identification of Potentially Tolerated Fish Species by Multiplex IgE Testing of a Multinational Fish-Allergic Patient Cohort.
%A Kalic T
%A Kuehn A
%A Aumayr M
%A Bartra J
%A Bindslev-Jensen C
%A Codreanu-Morel F
%A Domínguez O
%A Forstenlechner P
%A Hemmer W
%A Kamath SD
%A Leung A
%A Leung N
%A Lifanov Y
%A Mortz CG
%A Pascal M
%A Ristl R
%A Sørensen M
%A Üzülmez Ö
%A Yeghiazaryan L
%A Wong G
%A Hafner C
%A Breiteneder H
%J J Allergy Clin Immunol Pract
%V 10
%N 12
%D Dec 2022
%M 36002102
暂无%R 10.1016/j.jaip.2022.08.019
%X <B>BACKGROUND: </B>Although recent studies indicated that many fish-allergic patients may safely consume certain fish species, no clinical guidelines are available for identification of the exact species tolerated by specific patients.<BR><B>OBJECTIVE: </B>To investigate whether multiplex immunoglobulin E (IgE) testing reveals potentially tolerated fish through absence of IgE to parvalbumin (PV) and extracts from specific species.<BR><B>METHODS: </B>Sera from 263 clinically well-defined fish-allergic patients from Austria, China, Denmark, Luxembourg, Norway, and Spain were used in a research version of the ALEX2 multiplex IgE quantification assay. Specific IgE to PVs from 10 fish species (9 bony and 1 cartilaginous), and to extracts from 7 species was quantified. The IgE signatures of individual patients and patient groups were analyzed using SPSS and R.<BR><B>RESULTS: </B>Up to 38% of the patients were negative to cod PV, the most commonly used molecule in fish allergy diagnosis. Forty-five patients (17%) tested negative to PVs but positive to the respective fish extracts, underlining the requirement for extracts for accurate diagnosis. Between 60% (Spain) and 90% (Luxembourg) of the patients were negative to PV and extracts from ray, a cartilaginous fish, indicating its potential tolerance. Up to 21% of the patients were negative to at least 1 bony fish species. Of the species analyzed, negativity to mackerel emerged as the best predictive marker of negativity to additional bony fish, such as herring and swordfish.<BR><B>CONCLUSIONS: </B>Parvalbumins and extracts from multiple fish species relevant for consumption should be used in fish-allergy diagnosis, which may help identify potentially tolerated species for individual patients.