%0 Journal Article
%T Krüppel-like factor 5 rewires NANOG regulatory network to activate human naive pluripotency specific LTR7Ys and promote naive pluripotency.
%A Ai Z
%A Xiang X
%A Xiang Y
%A Szczerbinska I
%A Qian Y
%A Xu X
%A Ma C
%A Su Y
%A Gao B
%A Shen H
%A Bin Ramli MN
%A Chen D
%A Liu Y
%A Hao JJ
%A Ng HH
%A Zhang D
%A Chan YS
%A Liu W
%A Liang H
%A Ai Z
%A Xiang X
%A Xiang Y
%A Szczerbinska I
%A Qian Y
%A Xu X
%A Ma C
%A Su Y
%A Gao B
%A Shen H
%A Bin Ramli MN
%A Chen D
%A Liu Y
%A Hao JJ
%A Ng HH
%A Zhang D
%A Chan YS
%A Liu W
%A Liang H
%A Ai Z
%A Xiang X
%A Xiang Y
%A Szczerbinska I
%A Qian Y
%A Xu X
%A Ma C
%A Su Y
%A Gao B
%A Shen H
%A Bin Ramli MN
%A Chen D
%A Liu Y
%A Hao JJ
%A Ng HH
%A Zhang D
%A Chan YS
%A Liu W
%A Liang H
%J Cell Rep
%V 40
%N 8
%D Aug 2022 23
%M 36001968
暂无%R 10.1016/j.celrep.2022.111240
%X Endogenous retroviruses (ERVs) have been reported to participate in pre-implantation development of mammalian embryos. In early human embryogenesis, different ERV sub-families are activated in a highly stage-specific manner. How the specificity of ERV activation is achieved remains largely unknown. Here, we demonstrate the mechanism of how LTR7Ys, the human morula-blastocyst-specific HERVH long terminal repeats, are activated by the naive pluripotency transcription network. We find that KLF5 interacts with and rewires NANOG to bind and regulate LTR7Ys; in contrast, the primed-specific LTR7s are preferentially bound by NANOG in the absence of KLF5. The specific activation of LTR7Ys by KLF5 and NANOG in pluripotent stem cells contributes to human-specific naive pluripotency regulation. KLF5-LTR7Y axis also promotes the expression of trophectoderm genes and contributes to the expanded cell potential toward extra-embryonic lineage. Our study suggests that HERVs are activated by cell-state-specific transcription machinery and promote stage-specific transcription network and cell potency.