%0 Journal Article
%T Heterologous Expression and Purification of GPCRs.
%A Li X
%A Shen L
%A Liu J
%A Liu X
%A Liu ZJ
%A Hua T
%J Methods Mol Biol
%V 2507
%N 0
%D 2022
%M 35773588
暂无%R 10.1007/978-1-0716-2368-8_15
%X G protein-coupled receptors (GPCRs) are involved in a variety of human physiological processes and are attractive targets for treating various diseases. Yet, despite the importance as therapeutic targets, only 97 unique GPCR structures have been determined to date. A key challenge in their structural biology study is to obtain adequate protein samples because GPCRs usually have the low expression in native tissues. The in vitro recombinant expression provides the possibility to obtain large quantities of high-quality proteins suitable for three-dimensional structure determination by crystallography or single particle cryo-EM methods. For GPCR protein production, eukaryotic expression systems, such as baculovirus system and mammalian system, are the most widely used. In this chapter, we provide an overview of the methodological approaches on GPCRs expression and purification optimization using insect cells and mammalian cells, which is the prerequisite conditions for structural biology studies.