%0 Journal Article
%T Morphology of human fetal enteric neurons: A comparative study of different segments of the colon.
%A Baruhee D
%A Ganapathy A
%A Singh S
%A Sarwar S
%A Banerjee A
%A Bhukya S
%A Quadri JA
%A Shariff A
%J Morphologie
%V 107
%N 356
%D Mar 2023
%M 35764504
暂无%R 10.1016/j.morpho.2022.03.001
%X <B>OBJECTIVE: </B>The Enteric Nervous System (ENS) present in the wall of the gut is currently being explored because of its influence on the gut and beyond. In this context, the morphology of developing ENS has not been completely understood in humans due to lack of adequate literature. The aim of the present study was to observe the morphology of the enteric neurons in the human fetal colon and compare the findings in ascending colon a midgut derivative and descending colon a hindgut derivative at various weeks of gestation (WG).<BR><B>METHODS: </B>Tissue samples from 15 aborted fetuses (11 WG to 2 months postnatal) were processed for Cresyl violet, H & E staining, and NADPH Diaphorase histochemistry. The morphometric analysis was done by calculating the neuronal number density and neuronal fractional area. The Student t-test; Mann-Whitney test and Wilcoxon signed-rank test were used to analyze the data.<BR><B>RESULTS: </B>The muscularis externa with two distinct layers was visible as early as 13 WG and the muscularis mucosae was first observed at 18 WG. The size of the myenteric neurons appeared to be larger with increasing weeks of gestation suggesting a process of neuronal maturation. The neuronal number density and neuronal fractional area seemed to be reduced with advancing fetal age. There was no marked difference between the ascending and sigmoid colon. At 23 and 26 WG, a mature pattern of nitrergic innervation was observed.<BR><B>CONCLUSIONS: </B>This study is done on human fetal tissue samples unlike previous studies on animal samples to comprehend the morphology of developing ENS. It will aid in understanding the effect of ENS on various neurological disorders.