%0 Journal Article
%T Identification and characterization of a novel endo-β-1,4-glucanase from a soil metagenomic library.
%A Chai S
%A Zhang X
%A Gao Y
%A Nie L
%A Xiang C
%A Cao M
%A Wang S
%A Feng Z
%J Carbohydr Res
%V 510
%N 0
%D Dec 2021
%M 34700218
%F 2.975
%R 10.1016/j.carres.2021.108460
%X A cosmid clone cZFYN1413 with CMCase activity was identified from a soil metagenomic library. The sequence analysis of a subclone of cZFYN1413 revealed an endo-β-1,4-glucanase gene ZFYN1413 belonging to glycoside hydrolase family 6 and a transmembrane region in the N-terminal of ZFYN1413. Expression of ZFYN1413 in Escherichia coli BL21 (DE3) resulted in ZFYN1413-87, which was a truncated protein cleaved in transmembrane region of ZFYN1413. ZFYN1413-87 was expressed and its enzyme properties were studied. ZFYN1413-87 possessed strong endo-β-1,4-glucanase activity, and 52% of the activity could be retained after the protein was treated in buffer of pH 3.0 for 2 h. The study provided a special example of endo-β-1,4-glucanase in GH6 family.