%0 Journal Article
%T Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry.
%A Kamimura R
%A Uchida D
%A Kanno SI
%A Shiraishi R
%A Hyodo T
%A Sawatani Y
%A Shimura M
%A Hasegawa T
%A Tsubura-Okubo M
%A Yaguchi E
%A Komiyama Y
%A Fukumoto C
%A Izumi S
%A Fujita A
%A Wakui T
%A Kawamata H
%J Int J Mol Sci
%V 22
%N 20
%D Oct 2021 9
%M 34681573
%F 6.208
%R 10.3390/ijms222010913
%X TSC-22 (TGF-β stimulated clone-22) has been reported to induce differentiation, growth inhibition, and apoptosis in various cells. TSC-22 is a member of a family in which many proteins are produced from four different family genes. TSC-22 (corresponding to TSC22D1-2) is composed of 144 amino acids translated from a short variant mRNA of the TSC22D1 gene. In this study, we attempted to determine the intracellular localizations of the TSC22D1 family proteins (TSC22D1-1, TSC-22 (TSC22D1-2), and TSC22(86) (TSC22D1-3)) and identify the binding proteins for TSC22D1 family proteins by mass spectrometry. We determined that TSC22D1-1 was mostly localized in the nucleus, TSC-22 (TSC22D1-2) was localized in the cytoplasm, mainly in the mitochondria and translocated from the cytoplasm to the nucleus after DNA damage, and TSC22(86) (TSC22D1-3) was localized in both the cytoplasm and nucleus. We identified multiple candidates of binding proteins for TSC22D1 family proteins in in vitro pull-down assays and in vivo binding assays. Histone H1 bound to TSC-22 (TSC22D1-2) or TSC22(86) (TSC22D1-3) in the nucleus. Guanine nucleotide-binding protein-like 3 (GNL3), which is also known as nucleostemin, bound to TSC-22 (TSC22D1-2) in the nucleus. Further investigation of the interaction of the candidate binding proteins with TSC22D1 family proteins would clarify the biological roles of TSC22D1 family proteins in several cell systems.