%0 Journal Article
%T In vitro and in vivo activity of new strains of Bacillus subtilis against ESBL-producing Escherichia coli: an experimental study.
%A Ishnaiwer M
%A Bezabih Y
%A Javaudin F
%A Sassi M
%A Bemer P
%A Batard E
%A Dion M
%J J Appl Microbiol
%V 0
%N 0
%D Oct 2021 22
%M 34679216
%F 4.059
%R 10.1111/jam.15329
%X <B>OBJECTIVE: </B>The gastro-intestinal tract is a major reservoir of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli. Bacillus spores may be used as probiotics to decrease digestive colonization by ESBL-E. coli. Our aim was to assess the in vitro and in vivo activity of new Bacillus strains against ESBL-E. coli.<BR><B>RESULTS: </B>We screened the in vitro activity of 50 Bacillus strains against clinical isolates of ESBL-E. coli and selected B. subtilis strains CH311 and S3B. Both strains decreased ESBL-E. coli titers by 4 log10 CFU L-1 in an in vitro model of gut content, whereas the B. subtilis CU1 strain did not. In a murine model of intestinal colonization by ESBL-E. coli, CH311 and S3B did not decrease fecal titers of ESBL-E. coli. Ten sequences of putative antimicrobial peptides were identified in the genomes of CH311 and S3B, but not in CU1.<BR><B>CONCLUSIONS: </B>Two new B. subtilis strains showed strong in vitro activity against ESBL-E. coli.<BR><B>CONCLUSIONS: </B>Despite strong in vitro activities of new B. subtilis strains against ESBL-E. coli, intestinal colonisation was not altered by curative Bacillus treatment even if their spores proved to germinate in the gut. Thus, this work underlines the importance of in vivo experiments to identify efficient probiotics. The use of potential antimicrobial compounds identified by genome sequencing remains an attractive alternative to explore.