%0 Journal Article
%T Line-field confocal optical coherence tomography as a tool for three-dimensional in vivo quantification of healthy epidermis: A pilot study.
%A Chauvel-Picard J
%A Bérot V
%A Tognetti L
%A Orte Cano C
%A Fontaine M
%A Lenoir C
%A Pérez-Anker J
%A Puig S
%A Dubois A
%A Forestier S
%A Monnier J
%A Jdid R
%A Cazorla G
%A Pedrazzani M
%A Sanchez A
%A Fischman S
%A Rubegni P
%A Del Marmol V
%A Malvehy J
%A Cinotti E
%A Perrot JL
%A Suppa M
%J J Biophotonics
%V 0
%N 0
%D Oct 2021 4
%M 34608756
%F 3.39
%R 10.1002/jbio.202100236
%X Epidermal three-dimensional (3D) topography/quantification has not been completely characterized yet. The recently developed line-field confocal optical coherence tomography (LC-OCT) provides real-time, high-resolution, in-vivo 3D imaging of the skin. This pilot study aimed at quantifying epidermal metrics (epidermal thicknesses, dermal-epidermal junction [DEJ] undulation and keratinocyte number/shape/size) using 3D LC-OCT. For each study participant (8 female, skin-type-II, younger/older volunteers), seven body sites were imaged with LC-OCT. Epidermal metrics were calculated by segmentations and measurements assisted by artificial intelligence (AI) when appropriate. Thicknesses of epidermis/SC, DEJ undulation and keratinocyte nuclei volume varied across body sites. Evidence of keratinocyte maturation was observed in vivo: keratinocyte nuclei being small/spherical near the DEJ and flatter/elliptical near the skin surface. Skin microanatomy can be quantified by combining LC-OCT and AI. This technology could be highly relevant to understand aging processes and conditions linked to epidermal disorders. Future clinical/research applications are to be expected in this scenario.