%0 Journal Article
%T Engineering threshold-based selection systems.
%A Pedone KH
%A González-Pérez V
%A Leopold LE
%A Rasmussen NR
%A Der CJ
%A Cox AD
%A Ahmed S
%A Reiner DJ
%J G3 (Bethesda)
%V 11
%N 9
%D 09 2021 6
%M 34544135
%F 3.542
%R 10.1093/g3journal/jkab234
%X Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.