%0 Journal Article
%T Characterization of Gangliosides in Three Sea Urchin Species by HILIC-ESI-MS/MS.
%A Ma Y
%A Wang X
%A Wang Z
%A Cong P
%A Xu J
%A Xue C
%J J Agric Food Chem
%V 69
%N 27
%D Jul 2021 14
%M 34184526
%F 5.895
%R 10.1021/acs.jafc.1c02058
%X Sea urchin gangliosides (SU-GLSs) are well acknowledged for their nerve regeneration activity and neuroprotective property. The present study sought to characterize and semi-quantitate different SU-GLS subclasses in three sea urchin species, including Strongylocentrotus nudus, Hemicentrotus pulcherrimus, and Glyptocidaris crenularis. A total of 14 SU-GLS subclasses were identified by a hydrophilic interaction liquid chromatography-Q-Exactive tandem mass spectrometry method. Three sialic acid (Sia) structures, including Neu5Ac, Neu5Gc, and KDN, were identified in SU-GLSs, of which Neu5Ac and Neu5Gc had their corresponding sulfated forms. The linkage among Sias was determined to be 2-8. Additionally, KDN2-6Glc1-1Cer, KDN2-8Neu5Gc2-6Glc1-1Cer, and KDN2-8Neu5Gc2-8Neu5Gc2-6Glc-1Cer were speculated to be novel SU-GLS structures. Furthermore, the total SU-GLS content was 2.0-7.3 mg/g in the three sea urchin species. These results will provide useful data for developing a SU-GLS database of aquatic products. Besides, this study will provide a theoretical basis to explore the nutritional values of seafood products further.