%0 Journal Article
%T Genotypic characterization of carbapenem resistant Enterobacterales in clinical isolates from western Maharashtra.
%A Giri S
%A Karade S
%A Sen S
%J Indian J Med Microbiol
%V 39
%N 4
%D Oct-Dec 2021
%M 34016469
%F 1.347
%R 10.1016/j.ijmmb.2021.05.008
%X <B>OBJECTIVE: </B>To study distribution of carbapenemase genes namely; New Delhi metallo-beta-lactamase (blaNDM), Oxacillinase-48 (blaOXA48), Verona Integron-Encoded Metallo-beta-lactamase (blaVIM) and Imipenemase (blaIMP) in carbapenem resistant Enterobacterales (CRE), isolated from clinical samples.<BR><B>METHODS: </B>This cross-sectional study was conducted at a tertiary care hospital of western Maharashtra over six months period. CREs were identified by conventional disc diffusion and modified carbapenem inactivation method (mCIM). A total of 50 consecutive CRE isolates from clinical samples were subjected to home brewed polymerase chain reaction (PCR) for detection of carbapenemases.<BR><B>RESULTS: </B>Out of the 50 CRE isolates, at least one of the four carbapenemase genes was detected in 49 (98%) isolates. The frequency of distribution of these genes were NDM 90% (n = 45), OXA48 60% (n = 30) and VIM 12% (n = 6). Dual combination of blaNDM and blaOXA48 (50%) was the commonest pattern observed, which was frequently associated with Klebsiella pneumoniae.<BR><B>CONCLUSIONS: </B>The study indicate high prevalence of NDM warranting strict anti-microbial stewardship practices. Surveillance of CRE and resistance mechanism is essential to monitor the trend and take informed decision for appropriate anti-microbial therapy.