%0 Journal Article
%T An ionising radiation-induced specific transcriptional signature of inflammation-associated genes in whole blood from radiotherapy patients: a pilot study.
%A Cruz-Garcia L
%A Badie C
%A Anbalagan S
%A Moquet J
%A Gothard L
%A O'Brien G
%A Somaiah N
%A Ainsbury EA
%J Radiat Oncol
%V 16
%N 1
%D May 2021 3
%M 33941218
%F 4.309
%R 10.1186/s13014-021-01807-4
%X <B>BACKGROUND: </B>This communication reports the identification of a new panel of transcriptional changes in inflammation-associated genes observed in response to ionising radiation received by radiotherapy patients.<BR><B>METHODS: </B>Peripheral blood samples were taken with ethical approval and informed consent from a total of 20 patients undergoing external beam radiotherapy for breast, lung, gastrointestinal or genitourinary tumours. Nanostring nCounter analysis of transcriptional changes was carried out in samples prior and 24 h post-delivery of the 1st radiotherapy fraction, just prior to the 5th or 6th fraction, and just before the last fraction.<BR><B>RESULTS: </B>Statistical analysis with BRB-ArrayTools, GLM MANOVA and nSolver, revealed a radiation responsive panel of genes which varied by patient group (type of cancer) and with time since exposure (as an analogue for dose received), which may be useful as a biomarker of radiation response.<BR><B>CONCLUSIONS: </B>Further validation in a wider group of patients is ongoing, together with work towards a full understanding of patient specific responses in support of personalised approaches to radiation medicine.