%0 Journal Article
%T Vps34 Inhibits Hepatocellular Carcinoma Invasion by Regulating Endosome-Lysosome Trafficking via Rab7-RILP and Rab11.
%A Qi C
%A Zou L
%A Wang S
%A Mao X
%A Hu Y
%A Shi J
%A Zhang Z
%A Wu H
%J Cancer Res Treat
%V 0
%N 0
%D Mar 2021 26
%M 33781048
%F 5.036
%R 10.4143/crt.2020.578
%X <B>UNASSIGNED: </B>The role of Vps34, an indispensable protein required for cell vesicular trafficking, in the biological behavior of hepatocellular carcinoma (HCC) has yet to be studied.<BR><B>UNASSIGNED: </B>In the present study, the expression of Vps34 in HCC and the effect of Vps34 on HCC cell invasion was detected both in vivo and in vitro. Furthermore, by modulating the RILP and Rab11, which regulate juxtanuclear lysosome aggregation and recycling endosome respectively, the underlying mechanism was investigated.<BR><B>UNASSIGNED: </B>Vps34 was significantly decreased in HCC and negatively correlated with the HCC invasiveness both in vivo and in vitro. Moreover, Vps34 could promote lysosomal juxtanuclear accumulation, reduce the invasive ability of HCC cells via the Rab7-RILP pathway. In addition, the deficiency of Vps34 in HCC cells affected the endosome-lysosome system, resulting in enhanced Rab11 mediated endocytic recycling of cell surface receptor and increased invasion of HCC cells.<BR><B>UNASSIGNED: </B>Our study reveals that Vps34 acts as an invasion suppressor in HCC cells, and more importantly, the endosome-lysosome trafficking regulated by Vps34 has the potential to become a target pathway in HCC treatment.