%0 Case Reports
%T Direct Tissue PCR and Genotyping for Species Identification in a Case of Laryngeal Blastomycosis.
%A Fritsche TR
%A Anderson JL
%A Bassi D
%A Hall MC
%A Boyle TR
%A Meece JK
%J Ear Nose Throat J
%V 102
%N 4
%D Apr 2023 8
%M 33683980
%F 1.677
%R 10.1177/0145561321991342
%X Otolaryngologic manifestations of infection with Blastomyces species are extremely rare and restricted geographically to recognized endemic regions. Here, we describe a case of laryngeal blastomycosis that presented as slowly progressive dysphonia. While a preliminary diagnosis was made using routine histopathology, a species identification of Blastomyces dermatitidis was made using polymerase chain reaction amplification and rapid genotyping without the need for fungal culture. All symptoms resolved following 1 month of antifungal therapy. Rapid molecular differentiation of B dermatitidis from Blastomyces gilchristii provides important insights into pathogenesis given recent recognition of differences in clinical spectra.