%0 Journal Article
%T Isolation and Characterization of Salmonella Jumbo-Phage pSal-SNUABM-04.
%A Kwon J
%A Kim SG
%A Kim HJ
%A Giri SS
%A Kim SW
%A Lee SB
%A Park SC
%J Viruses
%V 13
%N 1
%D 12 2020 25
%M 33375688
%F 5.818
%R 10.3390/v13010027
%X The increasing emergence of antimicrobial resistance has become a global issue. Therefore, many researchers have attempted to develop alternative antibiotics. One promising alternative is bacteriophage. In this study, we focused on a jumbo-phage infecting Salmonella isolated from exotic pet markets. Using a Salmonella strain isolated from reptiles as a host, we isolated and characterized the novel jumbo-bacteriophage pSal-SNUABM-04. This phage was investigated in terms of its morphology, host infectivity, growth and lysis kinetics, and genome. The phage was classified as Myoviridae based on its morphological traits and showed a comparatively wide host range. The lysis efficacy test showed that the phage can inhibit bacterial growth in the planktonic state. Genetic analysis revealed that the phage possesses a 239,626-base pair genome with 280 putative open reading frames, 76 of which have a predicted function and 195 of which have none. By genome comparison with other jumbo phages, the phage was designated as a novel member of Machinavirus composed of Erwnina phages.