%0 Journal Article
%T In Vitro Production of Ergothioneine Isotopologues.
%A Beliaeva MA
%A Burn R
%A Lim D
%A Seebeck FP
%J Angew Chem Int Ed Engl
%V 60
%N 10
%D 03 2021 1
%M 32996678
%F 16.823
%R 10.1002/anie.202011096
%X Ergothioneine is an emerging component of the redox homeostasis system in human cells and in microbial pathogens, such as Mycobacterium tuberculosis and Burkholderia pseudomallei. The synthesis of stable isotope-labeled ergothioneine derivatives may provide important tools for deciphering the distribution, function, and metabolism of this compound in vivo. We describe a general protocol for the production of ergothioneine isotopologues with programmable 2 H, 15 N, 13 C, 34 S, and 33 S isotope labeling patterns. This enzyme-based approach makes efficient use of commercial isotope reagents and is also directly applicable to the synthesis of radio-isotopologues.