%0 Journal Article
%T Yersinia YopT inhibits RLH-mediated NF-κB and IRF3 signal transduction.
%A Li D
%A Wang H
%A Zhang P
%A Zhang Y
%A He X
%A Zhong H
%A Guan K
%A Min M
%A Gao Q
%A Wei C
%J Microbiol Immunol
%V 64
%N 11
%D Nov 2020
%M 32902897
%F 2.962
%R 10.1111/1348-0421.12842
%X The Gram-negative bacterial pathogen Yersinia delivers six effector proteins into the host cells to block the host innate immune response. One of the effectors, YopT, is a potent cysteine protease that causes the disruption of the actin cytoskeleton to inhibit phagocytosis of the pathogen; however, its molecular mechanism and relevance to pathogenesis need further investigation. In this report, we show that RIG-I is a novel target of the YopT protein. Remarkably, YopT interacts with RIG-I and inhibits rat liver homogenate-mediated nuclear factor-κB and interferon regulatory factor-3 activation. Further studies revealed a YopT-dependent increase in the K48-polymerized ubiquitination of RIG-I. These findings suggest that YopT negatively regulates RIG-I-mediated cellular antibacterial response by targeting RIG-I.