%0 Journal Article
%T Transcriptional and Functional Analysis of CD1c+ Human Dendritic Cells Identifies a CD163+ Subset Priming CD8+CD103+ T Cells.
%A Bourdely P
%A Anselmi G
%A Vaivode K
%A Ramos RN
%A Missolo-Koussou Y
%A Hidalgo S
%A Tosselo J
%A Nuñez N
%A Richer W
%A Vincent-Salomon A
%A Saxena A
%A Wood K
%A Lladser A
%A Piaggio E
%A Helft J
%A Guermonprez P
%J Immunity
%V 53
%N 2
%D 08 2020 18
%M 32610077
%F 43.474
%R 10.1016/j.immuni.2020.06.002
%X Dendritic cells (DCs) are antigen-presenting cells controlling T cell activation. In humans, the diversity, ontogeny, and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DCs (called DC3s) as immediate precursors of inflammatory CD88-CD14+CD1c+CD163+FcεRI+ DCs. DC3s develop via a specific pathway activated by GM-CSF, independent of cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors. Like classical DCs but unlike monocytes, DC3s drove activation of naive T cells. In vitro, DC3s displayed a distinctive ability to prime CD8+ T cells expressing a tissue homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor β (TGF-β) signaling. In vivo, DC3s infiltrated luminal breast cancer primary tumors, and DC3 infiltration correlated positively with CD8+CD103+CD69+ tissue-resident memory T cells. Together, these findings define DC3s as a lineage of inflammatory DCs endowed with a strong potential to regulate tumor immunity.