%0 Journal Article
%T Combining Phage Display and Next-Generation Sequencing for Materials Sciences: A Case Study on Probing Polypropylene Surfaces.
%A Juds C
%A Schmidt J
%A Weller MG
%A Lange T
%A Beck U
%A Conrad T
%A Börner HG
%J J Am Chem Soc
%V 142
%N 24
%D 06 2020 17
%M 32460497
%F 16.383
%R 10.1021/jacs.0c03482
%X Phage display biopanning with Illumina next-generation sequencing (NGS) is applied to reveal insights into peptide-based adhesion domains for polypropylene (PP). One biopanning round followed by NGS selects robust PP-binding peptides that are not evident by Sanger sequencing. NGS provides a significant statistical base that enables motif analysis, statistics on positional residue depletion/enrichment, and data analysis to suppress false-positive sequences from amplification bias. The selected sequences are employed as water-based primers for PP-metal adhesion to condition PP surfaces and increase adhesive strength by 100% relative to nonprimed PP.