%0 Journal Article
%T Effect of cell-extracellular matrix interaction on myogenic characteristics and artificial skeletal muscle tissue.
%A Ding R
%A Horie M
%A Nagasaka S
%A Ohsumi S
%A Shimizu K
%A Honda H
%A Nagamori E
%A Fujita H
%A Kawamoto T
%J J Biosci Bioeng
%V 130
%N 1
%D Jul 2020
%M 32278672
%F 3.185
%R 10.1016/j.jbiosc.2020.02.008
%X Although various types of artificial skeletal muscle tissue have been reported, the contractile forces generated by tissue-engineered artificial skeletal muscles remain to be improved for biological model and clinical applications. In this study, we investigated the effects of extracellular matrix (ECM) and supplementation of a small molecule, which has been reported to enhance α7β1 integrin expression (SU9516), on cell migration speed, cell fusion rate, myoblast (mouse C2C12 cells) differentiation and contractile force generation of tissue-engineered artificial skeletal muscles. When cells were cultured on varying ECM coated-surfaces, we observed significant enhancement in the migration speed, while the myotube formation (differentiation ratio) decreased in all except for cells cultured on Matrigel coated-surfaces. In contrast, SU9516 supplementation resulted in an increase in both the myotube width and differentiation ratio. Following combined culture with a Matrigel-coated surface and SU9516 supplementation, myotube width was further increased. Additionally, contractile forces produced by the tissue-engineered artificial skeletal muscles was augmented following combined culture. These findings indicate that regulation of the cell-ECM interaction is a promising approach to improve the function of tissue-engineered artificial skeletal muscles.