%0 Journal Article
%T Developmental patterns of enzyme activity, gene expression, and sugar content in sucrose metabolism of two broomrape species.
%A Farrokhi Z
%A Alizadeh H
%A Alizadeh H
%J Plant Physiol Biochem
%V 142
%N 0
%D Sep 2019
%M 31247445
%F 5.437
%R 10.1016/j.plaphy.2019.06.014
%X A better understanding of broomrape physiological features opens up new perspectives for developing specific management strategies. For this purpose, activities of key enzymes involved in osmoregulation (SAI1, CWI, M6PR, and SUS1) were considered at developmental stages of two important broomrape species (Egyptian and branched broomrape) on tomato. While Egyptian broomrape tubercles had high activities of invertases, branched broomrape shoots revealed high activities of M6PR and SUS1 during both pre- and post-emergence stages except for M6PR at post-emergence stages of P. aegyptiaca. Interestingly, the main accumulation of total reducing sugars was detected in tubercle during pre- and in shoot during post-emergence. Unlike low levels of genes expression (except for CWI) before parasite emergence, significantly higher expression levels of SAI1, SUS1 and M6PR were detected after parasite emergence. Matching the expression levels of SAI1 and SUS1 genes with their corresponding enzymes activities makes them as the suitable candidates for gene silencing strategies.