%0 Journal Article
%T A Fast and Reliable Method to Isolate Human Placental Macrophages.
%A Mezouar S
%A Ben Amara A
%A Chartier C
%A Gorvel L
%A Mege JL
%J Curr Protoc Immunol
%V 125
%N 1
%D 06 2019
%M 31125190
暂无%R 10.1002/cpim.77
%X Macrophages are specialized cells involved in recognition, uptake, and destruction of microorganisms. Human placental macrophages are poorly investigated because of the lack of a convenient protocol for their isolation. Here, we present a straightforward and reliable method to isolate macrophages from full-term human placentas. After enzymatic digestion of placental tissue and centrifugation of the cell suspension on a Ficoll cushion, placental macrophages are selected using magnetic beads coated with anti-CD14 antibodies. Isolated cells are characterized by flow cytometry. Ninety eight percent of isolated CD14+ placental macrophages also express the macrophage marker CD68. Thus, this efficient and reliable method yields placental macrophages at high purity and sufficient quantity for functional studies. © 2019 by John Wiley & Sons, Inc.