%0 Journal Article
%T Novel Dimethylhydrazine-Derived Spirolactam Fluorescent Chemodosimeter for Tracing Basal Peroxynitrite in Live Cells and Zebrafish.
%A Liu C
%A Zhang X
%A Li Z
%A Chen Y
%A Zhuang Z
%A Jia P
%A Zhu H
%A Yu Y
%A Zhu B
%A Sheng W
%J J Agric Food Chem
%V 67
%N 22
%D Jun 2019 5
%M 31083940
%F 5.895
%R 10.1021/acs.jafc.9b01298
%X The precise cellular function of peroxynitrite (ONOO-) in biosystems remains elusive, primarily owing to being short of ultrasensitive techniques for monitoring its intracellular distribution. In this work, a novel rhodamine B cyclic 1,2-dimethylhydrazine fluorescent chemodosimeter RDMH-PN for highly specific and ultrasensitive monitoring of basal ONOO- in biosystems was rationally designed. The fluorescence titration experiments demonstrated that RDMH-PN was capable of quantitatively detecting 0-100 nM ONOO- (limit of detection = 0.68 nM). In addition, RDMH-PN has outstanding performances of ultrafast measurement, naked-eye detection, and preeminent selectivity toward ONOO- to accurately detect intracellular basal ONOO-. Finally, it has been confirmed that RDMH-PN could not only map the intracellular basal ONOO- level by inhibition tests but also trace the fluctuations of endogenous and exogenous ONOO- levels with diverse stimulations in live cells and zebrafish.