%0 Journal Article
%T In Vivo Functional Mapping of a Cortical Column at Single-Neuron Resolution.
%A Tischbirek CH
%A Noda T
%A Tohmi M
%A Birkner A
%A Nelken I
%A Konnerth A
%J Cell Rep
%V 27
%N 5
%D 04 2019 30
%M 31042460
暂无%R 10.1016/j.celrep.2019.04.007
%X The cerebral cortex is organized in vertical columns that contain neurons with similar functions. The cellular micro-architecture of such columns is an essential determinant of brain dynamics and cortical information processing. However, a detailed understanding of columns is incomplete, even in the best studied cortical regions, and mostly restricted to the upper cortical layers. Here, we developed a two-photon Ca2+-imaging-based method for the serial functional mapping of all pyramidal layers of the mouse primary auditory cortex at single-neuron resolution in individual animals. We demonstrate that the best frequency-responsive neurons are organized in all-layers-crossing narrow columns, with fuzzy boundaries and a bandwidth of about one octave. This micro-architecture is, in many ways, different from what has been reported before, indicating the region and stimulus specificity of functional cortical columns in vivo.