%0 Journal Article
%T Bringing benign ectomesenchymal odontogenic tumours to the lab: An in vitro study using an organotypic culture model.
%A Bastos VC
%A Pereira NB
%A Diniz MG
%A Andrade LO
%A Castro WH
%A Kitten GT
%A Gomez RS
%A Gomes CC
%J J Oral Pathol Med
%V 48
%N 2
%D Feb 2019
%M 30554445
%F 3.539
%R 10.1111/jop.12812
%X <B>BACKGROUND: </B>Benign neoplasms exhibit most of the cellular phenomena considered hallmarks of cancer, except the capacity to metastasize. Thus, the elucidation of the mechanisms associated with the progression of benign neoplasms may complement and clarify the mechanisms involved in carcinogenesis. Benign odontogenic tumours often result in facial deformities and morbidities, and have complex pathogenesis, mainly due to the diversity of interactions between the odontogenic epithelium and the ectomesenchyme. Primary cell culture of such tumours is not only difficult to be established and maintained, but also tumour cells lose characteristic cellular morphology. Considering gene expression, growth, migration, proliferation and cellular morphology are controlled by cell-cell interactions and cell-extracellular matrix interactions, cell culture in 3D substrates has gained space as a way to overcome some of the limitations of traditional monolayer cell culture systems.<BR><B>METHODS: </B>In this study, fragments obtained from mesenchymal odontogenic tumours were cultured in type I collagen scaffolds. Invasion tests were performed in these models, as well as phenotypic characterization of the cultured tumours.<BR><B>RESULTS: </B>The results obtained for the odontogenic myxoma and the cemento-ossifying fibroma demonstrate a good reproduction of the growth pattern of these tumours under ex vivo conditions. Microscopic evaluation showed maintenance of cell viability in the explants for more than 30 days, without the presence of necrosis.<BR><B>CONCLUSIONS: </B>This is the first study involving long-term 3D primary cultures of benign odontogenic tumours, which is expected to support complex approaches to cell and molecular biology, and to serve as an experimental model for testing molecular therapies.