%0 Journal Article
%T Establishment of a HEK293 cell line by CRISPR/Cas9-mediated luciferase knock-in to study transcriptional regulation of the human SREBP1 gene.
%A Li Z
%A Zhao J
%A Muhammad N
%A Wang D
%A Mao Q
%A Xia H
%J Biotechnol Lett
%V 40
%N 11
%D Dec 2018
%M 30232659
%F 2.716
%R 10.1007/s10529-018-2608-2
%X <B>OBJECTIVE: </B>To establish a HEK293 cell line with a luciferase knock-in reporter controlled by the endogenous SREBP1 promoter for investigating transcriptional regulation of the SREBP1 gene.<BR><B>RESULTS: </B>PCR confirmed the site-specific integration of a single copy of the exogenous luciferase gene into one allele of the genome and a 14 bp deletion of the targeted sequence in the other. Luciferase activity was directly correlated with the promoter activity of the endogenous SREBP1 gene in the HEK293-SREBP1-T2A-luciferase-KI cell line cell line.<BR><B>CONCLUSIONS: </B>We successfully generated a novel luciferase knock-in reporter system, which will be very useful for studying transcriptional regulation of the SREBP1 gene and for screening drugs or chemical molecules that regulate SREBP1 gene expression.