%0 Journal Article
%T Interferon-stimulated gene 60 (ISG60) constitutes a negative feedback loop in the downstream of TLR3 signaling in hCMEC/D3 cells.
%A Imaizumi T
%A Sassa N
%A Kawaguchi S
%A Matsumiya T
%A Yoshida H
%A Seya K
%A Shiratori T
%A Hirono K
%A Tanaka H
%J J Neuroimmunol
%V 324
%N 0
%D 11 2018 15
%M 30195920
%F 3.221
%R 10.1016/j.jneuroim.2018.08.016
%X Brain capillary endothelial cells are the component of blood brain barrier, and the first line of defense against viruses invading into brain. We demonstrate that treatment of hCMEC/D3 cells, a human brain capillary endothelial cell line, with a Toll-like receptor 3 (TLR3) agonist polyinosinic-polycytidylic acid (poly IC) induces the expression of interferon (IFN)-stimulated gene 60 (ISG60), and this reaction was mediated by IFN-β. Knockdown of ISG60 increased the poly IC-induced expression of IFN-β and an IFN-β-inducible chemokine CXCL10. This indicates that ISG60 constitutes a negative feedback loop in the downstream of TLR3/IFN-β. ISG60 in brain capillary endothelial cells may contribute to prevent excess immune reactions associated with viral infections.