%0 Evaluation Study
%T Quantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet.
%A Ommen P
%A Zobek N
%A Meyer RL
%J J Microbiol Methods
%V 141
%N 0
%D 10 2017
%M 28802722
%F 2.622
%R 10.1016/j.mimet.2017.08.003
%X Crystal violet staining is commonly used for quantification of biofilm formation, although it is highly toxic. Here we test safranin as a non-toxic replacement. Safranin staining provided similar results as crystal violet, but with higher reproducibility. We therefore recommend safranin staining for biofilm biomass quantification.