%0 Journal Article
%T Characterization of the B Cell Transcriptome Bound by RNA-Binding Proteins with iCLIP.
%A Díaz-Muñoz MD
%A Monzón-Casanova E
%A Turner M
%J Methods Mol Biol
%V 1623
%N 0
%D 2017
%M 28589356
暂无%R 10.1007/978-1-4939-7095-7_14
%X Posttranscriptional regulation of gene expression shapes the B cell transcriptome and controls messenger RNA (mRNA) translation into protein. Recent reports have highlighted the importance of RNA binding proteins (RBPs) for mRNA splicing, subcellular location, stability, and translation during B lymphocyte development, activation, and differentiation. Here we describe individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP) in primary lymphocytes, a method that maps RNA-protein interactions in a genome-wide scale allowing mechanistic analysis of RBP function. We discuss the latest improvements in iCLIP technology and provide some examples of how integration of the RNA-protein interactome with other high-throughput mRNA sequencing methodologies uncovers the important role of RBP-mediated RNA regulation in key biological cell processes.