%0 Journal Article
%T Interferometric Scattering Microscopy for the Study of Molecular Motors.
%A Andrecka J
%A Takagi Y
%A Mickolajczyk KJ
%A Lippert LG
%A Sellers JR
%A Hancock WO
%A Goldman YE
%A Kukura P
%J Methods Enzymol
%V 581
%N 0
%D 2016
%M 27793291
%F 1.682
%R 10.1016/bs.mie.2016.08.016
%X Our understanding of molecular motor function has been greatly improved by the development of imaging modalities, which enable real-time observation of their motion at the single-molecule level. Here, we describe the use of a new method, interferometric scattering microscopy, for the investigation of motor protein dynamics by attaching and tracking the motion of metallic nanoparticle labels as small as 20nm diameter. Using myosin-5, kinesin-1, and dynein as examples, we describe the basic assays, labeling strategies, and principles of data analysis. Our approach is relevant not only for motor protein dynamics but also provides a general tool for single-particle tracking with high spatiotemporal precision, which overcomes the limitations of single-molecule fluorescence methods.