%0 Journal Article
%T Synthesis of substrates for periodate-coupled assay of phospholipases C and sphingomyelinases.
%A Larsen KL
%A Andersen RJ
%A Brask J
%J Enzyme Microb Technol
%V 91
%N 0
%D Sep 2016
%M 27444331
%F 3.705
%R 10.1016/j.enzmictec.2016.06.003
%X A series of 4-nitrophenyl (pNP) and 4-methylumbelliferyl (4MU) substrate analogues of phosphatidyl choline (PC) and phosphatidic acid (PA) were synthesized from 4-bromo-1-butene by ether formation, olefin epoxidation and ring opening with the phosphate head group. The pNP PC analogue, 4-(4-nitrophenoxy)-2-hydroxy-butyl-1-phosphoryl choline (1) was evaluated in assays of fungal sphingomyelinases, also displaying phospholipase C activity. Reactions were terminated with a periodate-containing stop solution, leading to liberation of pNP, quantified spectrophotometrically in an end-point measurement. A kinetic evaluation of sphingomyelinases from Kionochaeta sp. and Penicillium emersonii showed relatively high KM and low kcat values for this substrate, limiting its practical applicability in assays with low sphingomyelinase concentrations.