%0 Journal Article
%T Molecular analysis for bacterial contamination in dental unit water lines.
%A Watanabe A
%A Tamaki N
%A Matsuyama M
%A Kokeguchi S
%J New Microbiol
%V 39
%N 2
%D Apr 2016
%M 27196554
%F 1.383
%R 
%X Bacterial contamination in dental unit waterlines (DUWLs) was evaluated by molecular techniques in addition to the conventional culture method. Water samples (n=8) from DUWLs were investigated for heterotrophic bacteria by culture method using R2A agar. The selected bacterial antibiotic-resistance genes and Legionella species-specific 16SrDNA were identified by PCR. The profiles of bacterial contamination in DUWLs were further identified by PCR-DGGE. In this study, no antibiotic-resistant or Legionella genes were detected. Polycyclic aromatic hydrocarbon-degrading bacterium, Novosphingobium sp. was the most prevalent in DUWLs. Conventional PCR and PCR-DGGE were shown to be potentially useful for monitoring of bacterial contamination in DUWLs.