%0 Journal Article
%T Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry.
%A Vogel M
%A Suess B
%J Methods Mol Biol
%V 1380
%N 0
%D 2016
%M 26552820
暂无%R 10.1007/978-1-4939-3197-2_9
%X Isothermal titration calorimetry (ITC) is a powerful label-free technique to determine the binding constant as well as thermodynamic parameters of a binding reaction and is therefore well suited for the analysis of small molecule-RNA aptamer interaction. We will introduce you to the method and present a protocol for sample preparation and the calorimetric measurement. A detailed note section will point out useful tips and pitfalls.