%0 Journal Article
%T Nucleic acid from beans extracted by ethanediamine magnetic particles.
%A Qie F
%A Zhang G
%A Hou J
%A Sun X
%A Luo SZ
%A Tan T
%J J Food Sci Technol
%V 52
%N 3
%D Mar 2015
%M 25745257
%F 3.117
%R 10.1007/s13197-013-1168-7
%X Ethanediamine magnetite nanoparticles (EDAMPs) were used as adsorbents to isolate genomic DNA from various bean-species. A "single-pot" preparation of EDAMPs was described. Further characterization, including transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), thermo-gravimetric analysis (TGA), X-ray powder diffraction (XRD) and Fourier Transform Infrared Spectrophotometer (FT-IR) were used to demonstrate the efficiency of this simple and general identification method. The EDAMPs provided excellent yields of genomic DNA. The isolated DNA was suitable for use in further applications by the Polymerase Chain Reaction (PCR) for the detection of Genetically Modified (GM) food and non-GM food. The EDAMPs are effective for bioseparation applications.