%0 Journal Article
%T Proteomics of endosomal compartments from plants case study: isolation of trans-Golgi network vesicles.
%A Park E
%A Drakakaki G
%J Methods Mol Biol
%V 1209
%N 0
%D 2014
%M 25117284
暂无%R 10.1007/978-1-4939-1420-3_14
%X A detailed understanding of endomembrane processes and their biological roles is vital for a complete picture of plant growth and development; however their highly dynamic nature has complicated comprehensive and rigorous studies so far. Recent pioneering efforts have demonstrated that isolation of vesicles in their native state, paired with a quantitative identification of their cargo, offers a viable and practicable approach for the dissection of endomembrane trafficking pathways. The protocol presented in this chapter describes in detail the isolation of the SYP61 trans-Golgi network vesicles from Arabidopsis. With minor alterations, in a few key parameters, it can be adopted to yield a universal procedure for the broad spectrum of plant vesicles.