%0 Journal Article
%T High-resolution melting analysis for accurate detection of BRAF mutations: a systematic review and meta-analysis.
%A Chen D
%A Wang YY
%A Chuai ZR
%A Huang JF
%A Wang YX
%A Liu K
%A Zhang LQ
%A Yang Z
%A Shi DC
%A Liu Q
%A Huang Q
%A Fu WL
%J Sci Rep
%V 4
%N 0
%D Feb 2014 25
%M 24566771
%F 4.996
%R 10.1038/srep04168
%X The high-resolution melting curve analysis (HRMA) might be a good alternative method for rapid detection of BRAF mutations. However, the accuracy of HRMA in detection of BRAF mutations has not been systematically evaluated. We performed a systematic review and meta-analysis involving 1324 samples from 14 separate studies. The overall sensitivity of HRMA was 0.99 (95% confidence interval (CI) = 0.75-0.82), and the overall specificity was very high at 0.99 (95% CI = 0.94-0.98). The values for the pooled positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were 68.01 (95% CI = 25.33-182.64), 0.06 (95% CI = 0.03-0.11), and 1263.76 (95% CI = 393.91-4064.39), respectively. The summary receiver operating characteristic curve for the same data shows an area of 1.00 and a Q* value of 0.97. The high sensitivity and specificity, simplicity, low cost, less labor or time and rapid turnaround make HRMA a good alternative method for rapid detection of BRAF mutations in the clinical practice.