%0 Journal Article
%T A preliminary study on the radiation-resistance mechanism in ovarian cancer.
%A Liao Q
%A Zhang HM
%A Li HH
%A Zhou R
%A Mao HL
%A Chen YB
%A Cui MH
%J J Cancer Res Ther
%V 9
%N 1
%D Jan-Mar 2013
%M 23575069
%F 1.331
%R 10.4103/0973-1482.110346
%X <B>OBJECTIVE: </B>The present study was designed to explore the radiation-resistance mechanism by interfering in checkpoints kinase 1 (CHK1) and DNA-activated protein kinase (DNA-PK) genes with short hairpin RNA (shRNA) transfection into Skov3 cells derived from ovarian cancer and HeLa cells derived from cervical cancer.<BR><B>METHODS: </B>The cultured Skov3 and HeLa cells were transfected with plasmid vectors containing CHK1 shRNA and DNA-PK shRNA, respectively, through Lipofectimine™ 2000 mediation, and cultured for 20 hours before exposure to 2 Gy X-radiation. The cells were harvested 4 and 28 after X-irradiation respectively then washed 3 times with PBS. These cells were stained with Annexin V/PI and applied by flow cytometer to analyze alteration of apoptosis with software CellQuest.<BR><B>RESULTS: </B>The apoptotic response in Skov3 cells to X-radiation was significantly lower than that in HeLa cells at 4 hour (t = 15.22, P < 0.001) and 28 hours (t = 15.78, P < 0.001) of post-irradiation. The shRNA might not affect the apoptosis of Skov3 and HeLa cells, while shRNA-transfection significantly enhanced the apoptotic response in Skov3 cells to X-radiation as compared with that in HeLa cells.<BR><B>CONCLUSIONS: </B>The present work suggests that the CHK1 and DNA-PK genes are very likely to play a role in developing a radiation resistance in ovarian cancer.