%0 Journal Article
%T Analysis of arsenical metabolites in biological samples.
%A Hernandez-Zavala A
%A Drobna Z
%A Styblo M
%A Thomas DJ
%J Curr Protoc Toxicol
%V 42
%N 0
%D Nov 2009
%M 20396652
暂无%R 10.1002/0471140856.tx0433s42
%X Quantitation of iAs and its methylated metabolites in biological samples provides dosimetric information needed to understand dose-response relations. Here, methods are described for separation of inorganic and mono-, di-, and trimethylated arsenicals by thin layer chromatography. This method has been extensively used to track the metabolism of the radionuclide [(73)As] in a variety of in vitro assay systems. In addition, a hydride generation-cryotrapping-gas chromatography-atomic absorption spectrometric method is described for the quantitation of arsenicals in biological samples. This method uses pH-selective hydride generation to differentiate among arsenicals containing trivalent or pentavalent arsenic.