%0 Case Reports
%T Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: a case report.
%A Kordus RJ
%A Price RL
%A Davis JM
%A Whitman-Elia GF
%J J Assist Reprod Genet
%V 25
%N 9
%D Sep-Oct 2008
%M 18855132
%F 3.357
%R 10.1007/s10815-008-9254-x
%X <B>OBJECTIVE: </B>To describe the ultrastructure of spermatozoa from a patient with complete asthenozoospermia that resulted in live births following blastocyst culture.<BR><B>METHODS: </B>Analyses of spermatozoa from a 36 year old patient were performed using light and electron microscopy. The hypo-osmotic swelling test was used to select spermatozoa for intracytoplasmic sperm injection. Embryos were cultured to the blastocyst stage.<BR><B>RESULTS: </B>100% of the spermatozoa had dynein arm deficiency with secondary defects varying from 3-17%. Six oocytes were injected; five fertilized normally and one was digynic. All five zygotes formed good quality blastocysts. Three blastocysts were cryopreserved and two blastocysts were transferred. Twin females were born at 37 weeks.<BR><B>CONCLUSIONS: </B>The hypo-osmotic swelling test can be used to select viable immotile ejaculated spermatozoa from a patient with dynein arm deficiency and can produce excellent fertilization rates and blastocyst development resulting in live births.