%0 Journal Article
%T A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans.
%A Lacourse EJ
%A Perally S
%A Hernandez-Viadel M
%A Wright HA
%A Brophy PM
%J J Proteome Res
%V 7
%N 8
%D Aug 2008
%M 18582093
%F 5.37
%R 10.1021/pr8001035
%X Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations, protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans.