%0 Journal Article
%T Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas.
%A Uchiyama M
%A Maesawa C
%A Yashima-Abo A
%A Tarusawa M
%A Satoh M
%A Satoh T
%A Ishida Y
%A Ito S
%A Murai K
%A Enomoto S
%A Utsugisawa T
%A Masuda T
%J Lab Invest
%V 84
%N 7
%D Jul 2004
%M 15034598
%F 5.502
%R 10.1038/labinvest.3700092
%X We used 3'-minor groove binder (MGB) technology to develop consensus fluorogenically labeled probes of the immunoglobulin heavy-chain (IgH) gene for detecting minimal residual disease (MRD) in B-cell non-Hodgkin lymphoma (B-NHL). Sequence data from 59 patients with B-NHLs revealed a narrow consensus region as a result of somatic hypermutations and variable VH usage, indicating that it would be difficult to design ordinary non-MGB probes. MGB probes, characterized by shorter length but higher melting temperature, are more suitable for this situation than ordinary non-MGB probes. In fact, the present data indicated that about 20% more cases were detectable with MGB probes (34/59, 57.6%) than with the non-MGB probes (23/59, 39.0%) designed by Donovan et al. MGB technology is useful for the design of consensus fluorogenically labeled probes of the IgH gene for detecting MRD.