%0 Journal Article
%T A kinetic study on the interaction between tazobactam (a penicillanic acid sulphone derivative) and active-site serine beta-lactamases.
%A Perilli M
%A Franceschini N
%A Bonfiglio G
%A Segatore B
%A Stefani S
%A Nicoletti G
%A Perez MM
%A Bianchi C
%A Zollo A
%A Amicosante G
%J J Enzyme Inhib
%V 15
%N 1
%D 2000
%M 10850951
暂无%X The interaction between tazobactam and several chromosome- and plasmid-encoded (TEM, SHV, PSE types) class A and C beta-lactamases was studied by spectrophotometry. Tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner beta-lactam. A detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum beta-lactamases; the presence of a turn-over of acyl-enzyme complex was also evaluated.